Yo-Yo was the main subject and antagonist of the Annoying Orange episode Yo-Yo!. He made an abrupt entrance when he was tossed into the room by Dane Boedigheimer, smashing the Jello that Orange was arguing with earlier. Yo-Yo had felt very sick after the blow, making Orange think that he was a "barfing UFO". Yo-Yo had later recovered from his sickly state and informed Orange that he wasn't a UFO of any kind. He had also told Orange that he was a toy that did tricks while attached to a string. This made Orange immediately mistake him for a dog. Orange then began throwing a stick, a tennis ball, a frisbee and a dog biscuit in his general direction. Yo-Yo was then making an attempt to prove to Orange that he wasn't a dog (which was successful when Dane Boedigheimer began using him). Orange then warned him about Cuddles. This made Yo-Yo completely lose his temper, as he still thought that Orange was mistaking him for a dog, and also threatens to rip his eyes out. That is, until Cuddles grabs him and begins chewing him to bits and bits (much like she did to the trolls).